به نام خدا
Title: DNA extraction method with improved efficiency and specificity using DNA methyltransferase and click chemistry
Authors: Alexander B Artyukhin , YounHi Woo
Abstract: In an attempt to develop an alternative method to extract DNA from complex samples with much improved sensitivity and efficiency, here we report a proof-of-concept work for a new DNA extraction method using DNA methyltransferase (Mtase) and click chemistry. According to our preliminary data, the method has improved the current methods by (i) employing a DNA-specific enzyme, TaqI DNA Mtase, for improved selectivity, and by (ii) capturing the DNA through covalent bond to the functionalized surface, enabling a broad range of treatments yielding the final sample DNA with minimal loss and higher purity such that it will be highly compatible with downstream analyses. By employing Mtase, a highly DNA specific and efficient enzyme, and click chemistry, we demonstrated that as little as 0.1 fg of k-DNA (close to copy number 1) was captured on silica (Si)-based beads by forming a covalent bond between an azide group on the surface and the propargyl moiety on the DNA. This method holds promise in versatile applications where extraction of minute amounts of DNA plays critical roles such as basic and applied molecular biology research, bioforensic and biosecurity sciences, and state-of-the-art detection methods.
Publish Year: 2012
Published in: Analytical Biochemistry - Journal of Science Direct
Number of Pages: 6
موضوع: شیمی، بیوشیمی
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
به نام خدا
Title: The Current Status of Medical Physics in Asia-Oceania
Authors: TS Suh
Abstract: Asia has a diverse cultural and economical background. Asia-Oceania Federation of Organization for Medical Physics (AFOMP) was born in July 2000 during the World Congress 2000 in Chicago. The aim of AFOMP was to provide a solid relationship and useful information for closer collaboration and mutual support among the AFOMP members. The purpose of this presentation is to introduce the status of medical physics in Asia-Oceania providing the information of organization, regional congress, the status of medical physicist, etc. One of major role of AFOMP is to support the development of medical physics in Asia region. To improve the status of medical physicists in Asia, a goal-oriented action plans is required: especially, developing advancement of medical physics in developing countries, strengthening the education, training and professional development of medical physicists, and promoting good relations and the exchange of information with other international related organizations through regional congress and website etc.
Publish Year: 2009
Published in: IFMBE - Springer
Number of Pages: 2
موضوع: فیزیک پزشکی
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
به نام خدا
Title: A framework for enabling patient monitoring via mobile ad hoc network
Authors: Sweta Sneha, Upkar Varshney
Abstract: A critical component of comprehensive patient monitoring is reliability in communication between the patients and the healthcare professionals without any time and location dependencies. Patient monitoring applications largely rely on infrastructure based wireless networks for signal transmission. However, infrastructure based wireless networks till date, suffer from unpredictable network coverage and have thus been attributed to the unpredictable communication reliability of patient monitoring applications. This research investigates an approach based on leveraging mobile ad hoc network to address the challenge of enhancing communication reliability in the context of patient monitoring. Mobile ad hoc network, formed among patient monitoring devices, has the potential of enhancing network coverage and enabling signal transmission from an area which has low or non-existent coverage from infrastructure based networks. In order to utilize mobile ad hoc network in the context of patient monitoring we propose (1) power management protocols that address the challenge of managing the low battery power of patient monitoring devices while maximizing communication reliability and (2) a framework that models the complex decision logic involved in leveraging mobile ad hoc network for diverse patient monitoring scenarios. Analytical evaluation of the proposed approach supports the premise that mobile ad hoc network formed among patient monitoring devices can enhance the reliability of signal transmission thereby improving the quality of patient monitoring applications. Technical and managerial implications of the research findings and the direction of future research are discussed.
Publish Year: 2013
Published in: Decision Support Systems - Science Direct
Number of Pages: 17
موضوع: شبکه های Ad-Hoc
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
به نام خدا
Title: An efficient B + -tree design for main-memory database systems with strong access locality
Authors: PeiLun Suei a , Victor CS Lee b,fi , ShiWu Lo c , TeiWei Kuo
Abstract: This paper is motivated by the strong demands of many main-memory database applications with strong locality in data access, such as front-end logistical systems. We propose to adopt an auxiliary-tree approach with an tree-merging algorithm to efficiently handle bursty data insertions with keys in a small range and avoid significant overheads in tree rebalancing. A range-based deletion algorithm is then proposed to process data deletions with strong access locality in a batch fashion. The capability of the proposed approach is evaluated by a series of experiments with a wide range of workloads and a variety of locality patterns, where different tree index structures are compared in terms of the performance and memory space requirements.
Publish Year: 2013
Published in: Information Sciences - Science Direct
Number of Pages: 21
موضوع: پایگاه داده (Data Base )
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
به نام خدا
Title: Function optimisation by learning automata
Authors: QH Wu , HL Liao
Abstract: This paper presents a new algorithm, Function Optimisation by Learning Automata (FOLA), to solve complex function optimisation problems. FOLA consists of multiple automata, in which each automaton undertakes dimensional search on a selected dimension of the solution domain. A search action is taken on a path which is identified in the search space by the path value, and the path value is updated using the values of the states visited in the past, via a state memory that enables better use of the information collected in the optimisation process. In this paper, FOLA is compared with two popularly used particle swarm optimisers and four newly-proposed optimisers, on nine complex multi-modal benchmark functions. The experimental results have shown that in comparison with the other optimisers, FOLA offers better performance for most of the benchmark functions, in terms of its convergence rate and accuracy, and it uses much less computation time to obtain accurate solutions, especially for high-dimensional functions. In order to explore the FOLA s potential for applications, it is also applied to solve an optimal power flow problem of power systems. FOLA is able to minimise the fuel cost and enhance the voltage stability of the power system more efficiently in comparison with the other algorithms.
Publish Year: 2013
Published in: Information Sciences - Science Direct
Number of Pages: 20
موضوع: اتوماتای یادگیر (Learning Automata )
ایران سای – مرجع مقالات علمی فنی مهندسی
حامی دانش بومی ایرانیان
